851 Cancer associated fibroblasts in different T-stage lesions of cutaneous T-cell lymphoma

The tumor microenvironment (TME) has been shown to play an important role in initiation, development, and metastasis. Among all TME factors, cancer associated fibroblasts (CAF) have suggested a key role. Two studies reported that from cutaneous T-cell lymphoma (CTCL) lesions, mycosis fungoides (MF) and/or Sézary syndrome (SS), were hyper-activated exerted pro-tumorigenic activity on CTCL cells vitro. But much regarding CAFs development of remains be understood. In this study, we assessed different T-stage lesions (n=31) by immunohistochemistry using six CAF markers (FAP, PDGFRβ, S100A4, Vimentin, PDGFRα, α-SMA) comparison psoriatic (n=7) normal skins (n=4). FAP was expressed various fibroblasts, FAP+ increased compared skins. strongest staining seen T1 T4 lesions. Like FAP, PDGFRβ also but the PDGFRβ+ T2, T3, contrast, S100A4 Vimentin not only others including langerhans cells, macrophages, endothelial cells. S100A4+ Vimentin+ subset lymphocytes with atypical nuclei positive. T3 PDGFRα mainly round large skins/lesions, some keratinocytes expression α-SMA exclusively found vascular smooth muscle arrector mostly negative. There no difference numbers intensities α-SMA+ between or Our results suggest may useful are